ABSTRACT
Objective The aim of this study was to investigate the pulmonary function of healthy humans at middle and high altitudes using a 256-slice multidetector CT (MDCT) scanner.Methods We enrolled 40 healthy male volunteers aged 18－45 years in this study, 20 from the middle-altitude area (at a mean altitude of 2 260 m, the MA group) and the other 20 from the high-altitude area (at a mean altitude of 4 000 m, the HA group). Using 256-slice MDCT, we performed inspiratory and expiratory CT scanning of the lungs, analyzed the images with the GE (AW 4.6) Workstation software and collected such pulmonary function parameters as the mean lung density in the full inspiratory phase (MLDin) and expiratory phase (MLDex), lung volume in the full inspiratory phase (Vin) and expiratory phase (Vex), difference between Vin and Vex (Vin-Vex), and ratio of Vin to Vex (Vin/Vex), followed by comparison of the parameters between the two groups of subjects.Results Statistically significant differences were observed between the MA and HA groups in MLDex (\[-705.90±25.63] vs \[-745.50±12.76\] HU, P=0.000) but not in MLDin (\[-869.80±20.66\] vs \[-865.85±22.57\] HU, P=0.567). The Vex was markedly higher in the HA than in the MA group (\[2 279.59±520.25\] vs \[1 566.48±350.97\] mL, P<0.05) while both Vin-Vex and Vin/Vex were remarkably lower in the former than in the latter group (P<0.05).Conclusion CT quantitative technology may offer a deeper insight into human pulmonary function at a high altitude and provide some imaging evidence for the high-altitude medical explanation of the mechanisms underlying the adaptive capacity of human pulmonary function to hypoxic environment.
ABSTRACT
<p><b>OBJECTIVE</b>To establish the integrated discrete multiple organ cell culture (IdMOC) system.</p><p><b>METHODS</b>Rat primary cell of hepatocyte, nephrocyte, cardiomyocytes, alveolar macrophage, dermal fibroblasts were isolated by collagenase digestion, separation of bronchial lavage, two-step digestion method and cultured respectively, with monolayer culture. To establish the integrated discrete multiple organ cell culture (IdMOC) system, glass slides of five different cells were used to the same dish with 10% FBS DMEM medium cultured 7d, using MTT comparison primary cells cultured alone and cocultured when growth.</p><p><b>RESULTS</b>Established rat hepatocytes, renal cell, cardiomyocyte, alveolar macrophages, dermal fibroblasts separation method was stable, cell separation survival rate was about 90.0%. Hepatocytes separation survival rate 90.3% ,renal cell separation survival rate 91.9%, cardiomyocyte separation survival rate 93.0% and beating rate indifference curve among 3d-15d, alveolar macrophages cell separation survival rate 90.8%, dermal fibroblasts cell separation survival rate 92.7%. Five primary cells multiple organ cells coculture showed cocultured cell growth proliferation well, cultured alone and cocultured cells growth curve basic coincide.</p><p><b>CONCLUSION</b>Established rat multiple organ cell co-culture is successful.</p>